Headspace Solid Phase Micro Extraction Gas Chromatographic Determination of Fenthion in Human Serum

International Journal of Molecular Sciences

Konstantinos M. Kasiotis⁴  Helen Souki³  Angelos N. Tsakirakis⁴  Haris Carageorgiou³  Spiridon A. Theotokatos¹  Serkos A. Haroutounian² 

[1] Agroindustrial Robola Growers Cooperative of Kefalllinia, Omala, 28100 Kefallinia, Greece; E-mail:;Chemistry Laboratory, Agricultural University of Athens, Iera Odos 75, 118 55, Athens, Greece; E-mail:;Pharmacology Department, Medical School, University of Athens, 115 27, Athens, Greece; E-mail:;Benaki Phytopathological Institute, Laboratory of Pesticides Toxicology, 8 St. Delta Street, Kifissia 14561, Athens, Greece; E-mail:

关键词: Insecticide residue;    olive;    fenthion;    headspace;    SPME;    GC/MS;    metabolites;   

DOI  :  10.3390/ijms9050906

来源: mdpi

【 摘 要 】

A simple and effective analytical procedure was developed for the determination of fenthion residues in human serum samples. The sample treatment was performed using the headspace solid-phase micro extraction with polyacrylate fiber, which has the advantage to require low amount of serum (1 mL) without tedious pre-treatment. The quantification of fenthion was carried out by gas chromatography-mass spectrometry and the recoveries ranged from 79 to 104% at two spiking levels for 6 replicates. Detection and quantification limits were calculated as 1.51 and 4.54 ng/mL of serum respectively. Two fenthion metabolites fenoxon and fenthion–sulfoxide were also identified.

【 授权许可】

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