An Investigation on Micro-Raman Spectra and Wavelet Data Analysis for Pemphigus Vulgaris Follow-up Monitoring

Sensors

Carlo Camerlingo¹  Flora Zenone⁴  Giuseppe Perna³  Vito Capozzi³  Nicola Cirillo²  Giovanni Maria Gaeta² 

[1] Consiglio Nazionale delle Ricerche, Istituto di Cibernetica “E. Caianiello”, Pozzuoli, Italy;Dipartimento di Malattie Odontostomatologiche, Seconda Università di Napoli, Naples, Italy;Dipartimento di Scienze Biomediche, Università di Foggia, Italy;Dipartimento di Scienze Fisiche, Università “Federico II”, Naples, Italy

关键词: blood serum;    micro-Raman spectroscopy;    wavelet analysis;   

DOI  :  10.3390/s8063656

来源: mdpi

【 摘 要 】

A wavelet multi-component decomposition algorithm has been used for data analysis of micro-Raman spectra of blood serum samples from patients affected by pemphigus vulgaris at different stages. Pemphigus is a chronic, autoimmune, blistering disease of the skin and mucous membranes with a potentially fatal outcome. Spectra were measured by means of a Raman confocal microspectrometer apparatus using the 632.8 nm line of a He-Ne laser source. A discrete wavelet transform decomposition method has been applied to the recorded Raman spectra in order to overcome problems related to low-level signals and the presence of noise and background components due to light scattering and fluorescence. This numerical data treatment can automatically extract quantitative information from the Raman spectra and makes more reliable the data comparison. Even if an exhaustive investigation has not been done in this work, the feasibility of the follow-up monitoring of pemphigus vulgaris pathology has been clearly proved with useful implications for the clinical applications.

【 授权许可】

CC BY   
© 2008 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.