D-galactose/D-glucose-binding Protein from Escherichia coli as Probe for a Non-consuming Glucose Implantable Fluorescence Biosensor

Sensors

Viviana Scognamiglio¹  Vincenzo Aurilia¹  Nunzio Cennamo²  Paola Ringhieri¹  Luisa Iozzino¹  Micaela Tartaglia¹  Maria Staiano¹  Giuseppe Ruggiero¹  Pierangelo Orlando¹  Tullio Labella¹  Luigi Zeni²  Annalisa Vitale¹ 

[1] Laboratory for Molecular Sensing, IBP, CNR, Via Pietro Castellino, 111 80131 Naples, Italy;Department of Information Engineering, Seconda Università di Napoli, Italy

关键词: Biosensor;    Optical sensing;    Glucose;    Diabetes;    Fluorescence;    Proteins;   

DOI  :  10.3390/s7102484

来源: mdpi

【 摘 要 】

D-Galactose/D-glucose-binding protein from E. coli (GGBP) is a monomer that binds glucose with high affinity. The protein structure of GGBP is organized in two principal domains linked by a hinge region that form the sugar-binding site. In this work we show that the mutant form of GGBP at the amino acid position 182 can be utilized as a probe for the development of a non-consuming analyte fluorescence biosensor to monitor the glucose level in diabetes health care.

【 授权许可】

Unknown   
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