Dopamine-Imprinted Polymers: Template-Monomer Interactions, Analysis of Template Removal and Application to Solid Phase Extraction

Molecules

Piotr Luliński²  Dorota Maciejewska²  Magdalena Bamburowicz-Klimkowska¹ 

[1] Department of Toxicology, Faculty of Pharmacy, Medical University of Warsaw, Banacha 1 Str., 02-097 Warsaw, Poland;Department of Organic Chemistry, Faculty of Pharmacy, Medical University of Warsaw, Banacha 1 Str., 02-097 Warsaw, Poland

关键词: Dopamine hydrochloride isolation;    theoretical binding energy;    molecularly imprinted polymer;    template removal;    solid phase extraction;   

DOI  :  10.3390/12112434

来源: mdpi

【 摘 要 】

A dopamine-imprinted polymer (MIP) was prepared in aqueous methanol solution at 60^oC by free-radical cross-linking polymerization of methacrylic acid in the presence of ethylene glycol dimethacrylate as the cross-linker and dopamine hydrochloride as the template molecule. Its ability to isolate dopamine was evaluated as the basis of a solid phase extraction procedure and compared with that of a non-imprinted polymer (NIP). The binding of dopamine was 84.1% and 29.1% for MIP and NIP, respectively. Various reported post-polymerization treatments to reduce template bleeding were examined. In our case the lowest bleeding was achieved after applying a combined procedure: continuous extraction in a Soxhlet apparatus (CE), followed by microwave-assisted extraction (ME) to a level of 0.061 μg/mL. A simplified model of the template-monomer complexes allowed rationalization of monomer choice based on the heats of complex formation at a PM3 level of theory.

【 授权许可】

Unknown   
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