Inhibition of Acetylcholinesterase in Different Structures of the Rat Brain Following Soman Intoxication Pretreated with Huperzine A

International Journal of Molecular Sciences

Jiri Bajgar¹  Petr Hajek²  Jana Karasova¹  Dasa Slizova²  Otakar Krs²  Kamil Kuca¹  Daniel Jun¹  Josef Fusek¹ 

[1] Department of Toxicology, Faculty of Military Health Sciences, University of Defence, Trebesska 1575, 500 01 Hradec Kralove, Czech Republic;Department of Anatomy, Medical Faculty of Charles University, Hradec Kralove, Czech Republic

关键词: acetylcholinesterase;    rat brain parts;    soman;    Huperzine A;    pretreatment;   

DOI  :  10.3390/i8111165

来源: mdpi

【 摘 要 】

Acetylcholinesterase (AChE) activities in different brain parts were determined quantitatively in rats treated with huperzine A, soman, and huperzine A followed by soman, using histochemical and biochemical methods. Following soman intoxication (1.2 × LD₅₀, i.m.), AChE activity was decreased to 30–80% of control values depending on the brain structure. The most sensitive area was the frontal cortex and the most relatively resistant was ncl. ruber. Huperzine A treatment only caused a change in AChE activity varying from 70 to 100 % of control values. In rats pretreated with huperzine A and intoxicated with soman, AChE activity was significantly higher than that observed after soman. In these animals, survival of rats pretreated with huperzine was observed while the mortality of unpretreated animals was near to 80 %. The results suggest that huperzine A is good candidate for further study for clinical use as a prophylactic drug against nerve agent poisoning.

【 授权许可】

Unknown   
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