Combination of On-line pH and Oxygen Transfer Rate Measurement in Shake Flasks by Fiber Optical Technique and Respiration Activity MOnitoring System (RAMOS)

Sensors

Marco Scheidle¹  Johannes Klinger¹ 

[1] Biochemical Engineering, RWTH Aachen University, Sammelbau Biologie, Worringerweg 1, D-52074 Aachen, Germany E-mail

关键词: fiber optical pH measurement;    shaking flask;    oxygen transfer rate;    RAMOS;    E. coli;    process development;   

DOI  :  10.3390/s7123472

来源: mdpi

【 摘 要 】

Shake flasks are commonly used for process development in biotechnology industry. For this purpose a lot of information is required from the growth conditions during the fermentation experiments. Therefore, Anderlei et al. developed the RAMOS technology [1, 2], which proviedes on-line oxygen and carbondioxide transfer rates in shake flasks. Besides oxygen consumption, the pH in the medium also plays an important role for the successful cultivation of micro-organisms and for process development. For online pH measurement fiber optical methods based on fluorophores are available. Here a combination of the on-line Oxygen Transfer Rate (OTR) measurements in the RAMOS device with an on-line, fiber optical pH measurement is presented. To demonstrate the application of the combined measurement techniques, Escherichia coli cultivations were performed and on-line pH measurements were compared with off-line samples. The combination of on-line OTR and pH measurements gives a lot of information about the cultivation and, therefore, it is a powerful technique for monitoring shake flask experiments as well as for process development.

【 授权许可】

Unknown   
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