Isolation and Identification of an Anti-tumor Component from Leaves of Impatiens balsamina

Molecules

Zhi-Shan Ding¹  Fu-Sheng Jiang¹  Ni-Pi Chen¹  Gui-Yuan Lv¹ 

[1]Faculty of Life Science, Zhejiang Chinese Medical University, Hangzhou, 310053, P. R. China

[2] E-mails:

关键词: Impatiens balsamina;    anti-tumor;    MTT;    TLC;    2-methoxy-1;    4-naphthoquinone;   

DOI  :  10.3390/molecules13020220

来源: mdpi

【 摘 要 】

We have previously shown that ethanol or chloroform extracts of the leaves of Impatiens balsamina (LIB) have anti-tumor activity against the human hepatocellular carcinoma cell line HepG2. The ethanol extracts were separated into five fractions according to polarity. An MTT assay indicated that two of the fractions had anti-tumor activity and that the petroleum ether fraction (PEF) was the most active. But the available quantities of both the PEF and chloroform fractions (CHF) were limited, precluding further study. The chloroform extract (CHE) shared almost all the same spots with the PEF and CHF and was plentiful enough to carry out further separations. Thus, the CHE was further separated into six sub-fractions (CHE1~6) by column chromatography. A MTT assay showed that only the CHE2 fraction had a strong tumor inhibition ratio (IC₅₀ = 6.47±0.05 mg/L), which was superior to that of curcumin (IC₅₀ = 13.95±0.11 mg/L). However, TLC revealed that CHE2 was not pure and still contained two more components. After further separation and purification, followed by TLC and MTT assay confirmation, the final active component was isolated and identified as 2-methoxy-1,4-naphthoquinone by m.p., UV, MS and ¹³C- and ¹H-NMR data. This is the first report demonstrating that 2-methoxy-1,4-naphthoquinone has intensive in vitro anti-tumor activity against HepG2 cells.

【 授权许可】

Unknown   
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