Influence of Cadmium(II) Ions and Brewery Sludge on Metallothionein Level in Earthworms (Eisenia fetida) – Bio-transforming of Toxic Wastes

Sensors

Dalibor Huska³  Sona Krizkova³  Miroslava Beklova⁵  Ladislav Havel⁴  Josef Zehnalek³  Vaclav Diopan³  Vojtech Adam³  Ladislav Zeman²  Petr Babula¹ 

[1] Department of Natural Drugs, Faculty of Pharmacy and University of Veterinary and Pharmaceutical Sciences, Palackeho 1-3, CZ-612 42 Brno, Czech Republic;Department of Animal Nutrition and Forage Production, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Zemedelska 1, CZ-613 00 Brno, Czech Republic;Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Zemedelska 1, CZ-613 00 Brno, Czech Republic;Department of Plant Biology, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Zemedelska 1, CZ-613 00 Brno, Czech Republic;Department of Veterinary Ecology and Environmental Protection, Faculty of Veterinary Hygiene and Ecology, Faculty of Pharmacy and University of Veterinary and Pharmaceutical Sciences, Palackeho 1-3, CZ-612 42 Brno, Czech Republic

关键词: Metallothionein;    Biochemical marker;    Cadmium;    Heavy metals;    Earthworm;    Electrochemistry;    Voltammetry;   

DOI  :  10.3390/s8021039

来源: mdpi

【 摘 要 】

Metallothioneins belong to a group of intracellular, high molecular and cysteine-rich proteins whose content in an organism increase with increasing concentration of a heavy metal. The aim of this work was to apply the electrochemical analysis for the analysis of metallothioneins in earthworms exposed to cadmium ions and brewery sludge. Here we utilized adsorptive transfer technique coupled with differential pulse voltammetry Brdicka reaction to determine metallothionein in different biological samples. By means this very sensitive technique it was possible to analyze metallothionein in concentrations below 1 μmol.l⁻¹ with the standard deviation of 4-5%. We found out that the average MT level in the non-treated earthworms oscillated between 19 and 48 μmol.l⁻¹. When we analysed samples of earthworms treated by cadmium, we observed that the MT content increased with the exposition length and increase dose of cadmium ions. Finally, we attempted to study and compare the toxicity of the raw sludge and its leach by using of earthworms. The raw brewery sludge caused the death of the earthworms quickly. Earthworms held in the presence of leach from brewery sludge increased their weight of 147 % of their original weight because they ingested the nutrients from the sludge. The metallothionein level changes markedly with increasing time of exposition and applied dose of toxic compound. It clearly follows from the obtained results that the MT synthesis is insufficient in the first hours of the exposition and increases after more than 24 h.

【 授权许可】

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