Amperometric Low-Potential Detection of Malic Acid Using Single-Wall Carbon Nanotubes Based Electrodes

Sensors

Adina Arvinte¹  Lucian Rotariu¹ 

[1] id="af1-sensors-08-01497">Laboratory of Quality Control and Process Monitoring, Department of Analytical Chemistry, University of Bucharest, 4-12 Regina Elisabeta Blvd., 030018 Bucharest, Roman

关键词: Single wall carbon nanotubes;    electrode modification;    NADH;    malic acid;   

DOI  :  10.3390/s8031497

来源: mdpi

【 摘 要 】

The electrocatalytical property of single-wall carbon nanotube (SWNT) modified electrode toward NADH detection was explored by cyclic voltammetry and amperometry techniques. The experimental results show that SWNT decrease the overvoltage required for oxidation of NADH (to +300 mV vs. Ag/AgCl) and this property make them suitable for dehydrogenases based biosensors. The behavior of the SWNT modified biosensor for L-malic acid was studied as an example for dehydrogenases biosensor. The amperometric measurements indicate that malate dehydrogenase (MDH) can be strongly adsorbed on the surface of the SWNT-modified electrode to form an approximate monolayer film. Enzyme immobilization in Nafion membrane can increase the biosensor stability. A linear calibration curve was obtained for L-malic acid concentrations between 0.2 and 1mM.

【 授权许可】

Unknown   
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