RNA Interference in Mammalia Cells by RNA-3’-PNA Chimeras

International Journal of Molecular Sciences

Nicoletta Potenza²  Loredana Moggio¹  Giovanna Milano¹  Vincenzo Salvatore²  Benedetto Di Blasio¹  Aniello Russo² 

[1] Department of Environmental Sciences, Second University of Naples, Via Vivaldi 43, I-81100 Caserta, Italy; E-mail:;Department of Life Sciences, Second University of Naples, Via Vivaldi 43, I-81100 Caserta, Italy; E-mail:

关键词: modified siRNA;    RNA interference;    RNA-PNA chimeras;    Peptide Nucleic Acids;    RNA;   

DOI  :  10.3390/ijms9030299

来源: mdpi

【 摘 要 】

The discovery of siRNAs as the mediators of RNA interference has led to an increasing interest in their therapeutic applications. Chemical modifications are introduced into siRNAs to optimize the potency, the stability and the pharmacokinetic properties in vivo. Here, we synthesize and test the effects of RNA-3’-PNA chimeras on siRNA functioning and stability. We demonstrate that the chemical modifications are compatible with the siRNA machinery, because all the PNA-modified siRNAs can efficiently mediate specific gene silencing in mammalian cells. Furthermore, we find that the modification on the sense strand of siRNA results in an increased persistence of the activity, whereas modification on both strands results in enhanced nuclease resistance in serum.

【 授权许可】

CC BY   
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