Femtosecond Laser Microfabrication of an Integrated Device for Optical Release and Sensing of Bioactive Compounds

Sensors

Diego Ghezzi¹  Rebeca Martinez Vazquez²  Roberto Osellame²  Flavia Valtorta³  Alessandra Pedrocchi¹  Giuseppe Della Valle²  Roberta Ramponi²  Giancarlo Ferrigno¹ 

[1] NeuroEngineering and Medical Robotics Laboratory, Bioengineering Department, Politecnico di Milano, 20133 Milano, Italy. E-Mails:;Istituto di Fotonica e Nanotecnologie (IFN) - CNR, Dipartimento di Fisica, Politecnico di Milano, 20133 Milano, Italy. E-Mails:;San Raffaele Scientific Institute and Vita-Salute University, 20132 Milano, Italy. E-Mail:

关键词: Caged compound;    waveguides;    laser uncaging;    optical release;    femtosecond microfabrication;    optical sensing;   

DOI  :  10.3390/s8106595

来源: mdpi

【 摘 要 】

Flash photolysis of caged compounds is one of the most powerful approaches to investigate the dynamic response of living cells. Monolithically integrated devices suitable for optical uncaging are in great demand since they greatly simplify the experiments and allow their automation. Here we demonstrate the fabrication of an integrated bio-photonic device for the optical release of caged compounds. Such a device is fabricated using femtosecond laser micromachining of a glass substrate. More in detail, femtosecond lasers are used both to cut the substrate in order to create a pit for cell growth and to inscribe optical waveguides for spatially selective uncaging of the compounds present in the culture medium. The operation of this monolithic bio-photonic device is tested using both free and caged fluorescent compounds to probe its capability of multipoint release and optical sensing. Application of this device to the study of neuronal network activity can be envisaged.

【 授权许可】

CC BY   
© 2008 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.