Sensors | |
Determination of Vitamin C (Ascorbic Acid) Using High Performance Liquid Chromatography Coupled with Electrochemical Detection | |
Zbynek Gazdik3  Ondrej Zitka1  Jitka Petrlova1  Vojtech Adam1  Josef Zehnalek1  Ales Horna2  Vojtech Reznicek3  Miroslava Beklova4  | |
[1] Department of Chemistry and Biochemistry, Faculty of Agronomy, Zemedelska 1, CZ-613 00 Brno, Mendel University of Agriculture and Forestry, Czech Republic;Tomas Bata University, T.G. Masaryka 275, CZ-762 72 Zlin, Czech Republic;Department of Breeding and Propagation of Horticultural Plants, Faculty of Horticulture, Valticka 337, CZ-691 44 Lednice, Faculty of Agronomy, Zemedelska 1, CZ-613 00 Brno, Mendel University of Agriculture and Forestry, Czech Republic;Department of Veterinary Ecology and Environmental Protection, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Palackeho 1-3, CZ-612 42 Brno, Czech Republic | |
关键词: Ascorbic Acid; Flow Injection Analysis; High Performance Liquid Chromatography; Electrochemical Detection; Fruits; Pharmaceutical Preparation; Human Blood Serum; | |
DOI : 10.3390/s8117097 | |
来源: mdpi | |
【 摘 要 】
Vitamin C (ascorbic acid, ascorbate, AA) is a water soluble organic compound that participates in many biological processes. The main aim of this paper was to utilize two electrochemical detectors (amperometric – Coulouchem III and coulometric – CoulArray) coupled with flow injection analysis for the detection of ascorbic acid. Primarily, we optimized the experimental conditions. The optimized conditions were as follows: detector potential 100 mV, temperature 25 °C, mobile phase 0.09% TFA:ACN, 3:97 (
【 授权许可】
CC BY
© 2008 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.
【 预 览 】
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RO202003190058131ZK.pdf | 517KB | download |