Use of Liquid Chromatography with Electrochemical Detection for the Determination of Antioxidants in Less Common Fruits

Molecules

Zbynek Gazdik⁷  Vojtech Reznicek⁷  Vojtech Adam⁴  Ondrej Zitka⁴  Tunde Jurikova³  Boris Krska⁶  Jan Matuskovic⁵  Jan Plsek⁷  Jan Saloun¹  Ales Horna² 

[1] Department of Applied Pharmacy, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Palackeho 1 - 3, CZ-612 42 Brno, Czech Republic;Tomas Bata University, T.G. Masaryka 275, CZ-762 72 Zlin, Czech Republic;Institute of Natural and Informatics’ Sciences, Faculty of Central European Studies, Constantine the Philosopher University in Nitra, Nabrezie mladeze 91, SK-949 76 Nitra, Slovak Republic; E-mail:;Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Zemedelska 1, CZ-613 00 Brno, Czech Republic; E-mail:;Department of Fruit Production, Viticulture, and Enology, Horticulture and Landscape Engineering Faculty, University of Agriculture in Nitra, Trieda A. Hlinku 2, SK-949 76 Nitra, Slovak Republic;Department of Fruit Growing, Faculty of Horticulture, Mendel University of Agriculture and Forestry, Valtická 337, CZ-691 44 Lednice, Czech Republic,;Department of Breeding and Propagation of Horticultural Plants, Faculty of Horticulture, Mendel University of Agriculture and Forestry, Valtická 337, CZ-691 44 Lednice, Czech Republic; E-mails:

关键词: Phenolic compound;    Electrochemical detection;    Less common fruit species;    liquid Chromatography;    Neuroprotective food;   

DOI  :  10.3390/molecules131102823

来源: mdpi

【 摘 要 】

Neurodegenerative disorders (NDD) have become the common global health burden over the last several decades. According to World Health Organization (WHO), a staggering 30 million people will be affected by Alzheimer’s disease in Europe and the USA by 2050. Effective therapies in this complex field considering the multitude of symptoms associated with NDD indications, have not been found yet. Based on the results of NDD related studies, prevention appears to be the promise alternative. Antioxidative and anti-inflammatory properties are hypothesized for natural phenolics, a group of plant secondary products that may positively impact neurodegenerative diseases. In these studies, phenolic-rich extracts from less common fruit species: Blue honeysuckle (Lonicera edulis, Turcz. ex. Freyn), Saskatoon berry (Amelanchier alnifolia Nutt.), and Chinese hawthorn (Crateagus pinnatifida Bunge) were obtained and analyzed to detect neuroprotective substances content and establish a potential therapeutic value. High performance liquid chromatography with electrochemical detection was optimized and further applied on analysis of the extracts of less common fruit species. It was observed that Chinese hawthorn and Blue honeysuckle extracts are potent source of neuroprotective phenolic antioxidants. In accordance the results, it appears that the fruit or formulated products may have the potential for the prevention of neurodegenerative diseases.

【 授权许可】

CC BY   
© 2008 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.