Structure Determination of β-Glucans from Ganoderma lucidum with Matrix-assisted Laser Desorption/ionization (MALDI) Mass Spectrometry

Molecules

Wei-Ting Hung¹  Shwu-Huey Wang²  Chung-Hsuan Chen¹ 

[1] Genomics Research Center, Academia Sinica, No. 128, Academia Road Section 2, Nan-Kang, Taipei 11529, Taiwan;Instrument Center, Taipei Medical University, Taipei 11031, Taiwan

关键词: Polysaccharides;    matrix-assisted laser desorption/ionization (MALDI);    Ganoderma lucidum;    β-glucan;    2;    5-dihydroxybenzoic acid (2;    5-DHB).;   

DOI  :  10.3390/molecules13081538

来源: mdpi

【 摘 要 】

A novel method that uses matrix-assisted laser desorption/ionization (MALDI) mass spectrometry to analyze molecular weight and sequencing of glucan in Ganoderma lucidum is presented. Thus, β-glucan, which was isolated from fruiting bodies of G. lucidum, was measured in a direct and fast way using MALDI mass spectrometry. In addition, tandem mass spectrometry of permethylated glucans of G. lucidum, dextran, curdlan and maltohexaose were also pursued and different fragment patterns were obtained. The G. lucidum glucan structure was determined and this method for linkage analysis of permethylated glucan has been proven feasible.

【 授权许可】

CC BY   
© 2008 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.