A Novel Co-polymer Based on Hydroxypropyl α-Cyclodextrin Conjugated to Low Molecular Weight Polyethylenimine as an in Vitro Gene Delivery Vector

International Journal of Molecular Sciences

Hongliang Huang³  Hai Yu²  Da Li²  Yang Liu²  Fenping Shen²  Jun Zhou¹  Qingqing Wang² 

[1] Institute of Chemical Biology and Pharmaceutical Chemistry, Zhejiang University, Tianmushan Road 148, Hangzhou, P. R. China. E-Mail:;Institute of Immunology, Zhejiang University, Yuhangtang Road 388, Hangzhou, P. R. China. E-Mails:;School of Life Science, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, Guangzhou, P. R. China. E-Mail:

关键词: Polyethylenimine;    2-hydroxypropyl-α-cyclodextrin;    non-viral gene delivery vector;    co-polymer;   

DOI  :  10.3390/ijms9112278

来源: mdpi

【 摘 要 】

A novel co-polymer based on 2-hydroxypropyl-α-cyclodextrin cross-linked by low molecular weight polyethylenimine was synthesized as a gene delivery vector. The copolymer could bind and condense DNA tightly. It showed lower cytotoxicity than PEI 25kDa in SK-BR-3 cells. Transfection efficiency was increased over 5.5-fold higher than PEI 25 kDa in SK-BR-3 cells in complete serum medium. It is a potential candidate vector for gene therapy.

【 授权许可】

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