Diagnosis of Intoxication by the Organophosphate VX: Comparison Between an Electrochemical Sensor and Ellman´s Photometric Method

Sensors

Miroslav Pohanka¹  Martina Hrabinova² 

[1] Centre of Advanced Studies and Department of Toxicology, Faculty of Military Health Sciences, University of Defense / Trebesska 1575, 50001 Hradec Kralove, Czech Republic;

关键词: Organophosphate;    carbamate;    intoxication;    diagnosis;    biosensor;   

DOI  :  10.3390/s8095229

来源: mdpi

【 摘 要 】

An electrochemical sensor is introduced as a tool applicable for diagnosis of intoxication by cholinesterase inhibitors caused by the well-known nerve agent VX. The traditional Ellman method was chosen for comparison with the sensor's analytical parameters. Both methods are based on estimation of blood cholinesterase inhibition as a marker of intoxication. While Ellman's method provided a limit of detection of 5.2×10^-7 M for blood containing VX, the electrochemical sensor was able to detect 4.0×10^-7 M. Good correlation between both methods was observed (R = 0.92). The electrochemical sensor could be considered a convenient tool for a fast yet accurate method, easily available for field as well as laboratory use. Time and cost savings are key features of the sensor-based assay.

【 授权许可】

CC BY   
© 2008 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.