Inhibition of Citrinin Production in Penicillium citrinum Cultures by Neem [Azadirachta indica A. Juss (Meliaceae)]

International Journal of Molecular Sciences

Simone Aparecida Gallerani Mossini¹ 

[1] id="af1-ijms-09-01676">Department of Biochemistry, State University of Maringá. PR, Bras

关键词: Penicillium citrinum;    citrinin inhibition;    Azadirachta indica;    neem;   

DOI  :  10.3390/ijms9091676

来源: mdpi

【 摘 要 】

The efficacy of different concentrations of aqueous neem leaf extract (3.12 to 50 mg/mL) on growth and citrinin production in three isolates of Penicillium citrinum was investigated under laboratory conditions. Mycotoxin production by the isolates was suppressed, depending on the concentration of the plant extract added to culture media at the time of spore inoculation. Citrinin production in fungal mycelia grown for 21 days in culture media containing 3.12 mg/mL of the aqueous extract of neem leaf was inhibited by approximately 80% in three isolates of P. citrinum. High-performance liquid chromatography was performed to confirm the spectrophotometric results. Vegetative growth was assessed, but neem extract failed to inhibit it. Neem leaf extract showed inhibition of toxin production without retardation in fungal mycelia growth.

【 授权许可】

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