Isoquinoline Alkaloids Isolated from Corydalis yanhusuo and Their Binding Affinities at the Dopamine D1 Receptor

Molecules

Zhong-Ze Ma²  Wei Xu¹  Niels H. Jensen³  Bryan L. Roth³  Lee-Yuan Liu-Chen¹ 

[1] Department of Pharmacology and Center for Substance Abuse Research, School of Medicine, Temple University, 3420 N. Broad St, Philadelphia, PA 19140, USA; E-mail:;Bio-Organic and Natural Products Laboratory, McLean Hospital, Harvard Medical School, 115 Mill Street, Belmont, MA 02478, USA;Department of Pharmacology, University of North Carolina Medical School, Chapel Hill, NC 27599, USA; E-mail:

关键词: Corydalis yanhusuo;    Isoquinoline alkaloids;    d/l Ratio;    Chiral HPLC;    Dopamine receptor;   

DOI  :  10.3390/molecules13092303

来源: mdpi

【 摘 要 】

Bioactivity-guided fractionation of Corydalis yanhusuo has resulted in the isolation of eight known isoquinoline alkaloids - tetrahydropalmatine, isocorypalmine, stylopine, corydaline, columbamine, coptisin, 13-methylpalmatine, and dehydro-corybulbine. The tertiary alkaloids were further analyzed by chiral HPLC to determine the ratios of d-and l-isomers. The isolated compounds were screened for their binding affinities at the dopamine D₁ receptor. Isocorypalmine had the highest affinity (K_i = 83 nM). The structure-affinity relationships of these alkaloids are discussed.

【 授权许可】

CC BY   
© 2008 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.