Chemical Composition and Antimicrobial Properties of Piper ovatum Vahl

Molecules

Daniel Rodrigues Silva¹  Eliana Harue Endo¹  Benedito Prado Dias Filho¹  Celso Vataru Nakamura¹  Terezinha Inez Estivaleti Svidzinski²  Amanda de Souza³  Maria Claudia M. Young³  Tânia Ueda-Nakamura¹ 

[1] Programa de Pós-graduação em Ciências Farmacêuticas, Universidade Estadual de Maringá, Maringá, Paraná, Brazil;Departamento de Análises Clínicas, Universidade Estadual de Maringá, Maringá, Paraná, Brazil;Instituto de Botânica de São Paulo, São Paulo, SP, Brazil

关键词: Piper ovatum Vahl;    Chemical composition;    Essential oil;    Antimicrobial activity.;   

DOI  :  10.3390/molecules14031171

来源: mdpi

【 摘 要 】

The chemical composition of the essential oil obtained from the leaves of Piper ovatum Vahl by hydrodistillation was analyzed by GC–MS. The main constituents found were δ-amorphene (16.5 %), cis-muurola-4(14),5-diene (14.29 %) and γ-muurolene (13.26%). The crude extracts and isolated compounds were screened for their antimicrobial activity. Hydroalcoholic extracts of different parts of Piper ovatum Vahl, essential oil and amides isolated from leaves were tested against Gram-positive and Gram-negative bacteria and Candida species. All extracts and amides were active against Bacillus subtilis and Candida tropicalis, including clinical strains. Essential oil was active against C. tropicalis. These amides showed an inhibitory effect on the adherence of C. tropicalis ATCC 28707 on cover glasses at 10 µg/mL, but did not show morphological alterations at the tested concentrations. Amides were identified as piperovatine and piperlonguminine, and showed MIC values of 15.6 and 31.2 µg/mL to B. subtilis and 3.9 µg/mL to C. tropicalis, and low toxic effects to Vero cells and macrophages.

【 授权许可】

CC BY   
© 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.