Taiwanese Native Plants Inhibit Matrix Metalloproteinase-9 Activity after Ultraviolet B Irradiation

Molecules

Yueh-Lun Lee⁶  Mei-Hsien Lee¹  Hsiu-Ju Chang³  Po-Yuan Huang⁴  I-Jen Huang²  Kur-Ta Cheng⁵ 

[1] Graduate Institute of Pharmacognosy, Taipei Medical University, Taipei, Taiwan;Applied Bioscience Division, Taiwan Sugar Research Institute, Tainan, Taiwan;School of Nursing, Taipei Medical University, Taipei Taiwan;Graduate Institute of Cell and Molecular Biology, Taipei Medical University, Taipei, Taiwan;Department of Biochemistry, Taipei Medical University, Taipei, Taiwan;Department of Microbiology and Immunology, Taipei Medical University, Taipei, Taiwan

关键词: Taiwanese native plant;    Polyphenolic;    Matrix metalloproteinase-9.;   

DOI  :  10.3390/molecules14031062

来源: mdpi

【 摘 要 】

Medicinal plants have long been used as a source of therapeutic agents. They are thought to be important anti-aging ingredients in prophylactic medicines. The aim of this study was to screen extracts from Taiwanese plant materials for phenolic contents and measure the corresponding matrix metalloproteinase-9 (MMP-9) activity. We extracted biological ingredients from eight plants native to Taiwan (Alnus formosana, Diospyros discolor, Eriobotrya deflex, Machilus japonica, Pyrrosia polydactylis, Pyrus taiwanensis, Vitis adstricta, Vitis thunbergii). Total phenolic content was measured using the Folin-Ciocalteu method. MMP-9 activities were measured by gelatin zymography. The extracted yields of plants ranged from 3.7 % to 16.9 %. The total phenolic contents ranged from 25.4 to 36.8 mg GAE/g dry material. All of these extracts (except Vitis adstricta Hance) were shown to inhibit MMP-9 activity of WS-1 cell after ultraviolet B irradiation. These findings suggest that total phenolic content may influence MMP-9 activity and that some of the plants with higher phenolic content exhibited various biological activities that could serve as potent inhibitors of the ageing process in the skin. This property might be useful in the production of cosmetics.

【 授权许可】

CC BY   
© 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.