Biotransformation of Ginsenoside Rf to Rh1 by Recombinant β-Glucosidase

Molecules

Chang-Chun Ruan⁴  Hao Zhang¹  Lian-Xue Zhang⁴  Zhi Liu³  Guang-Zhi Sun³  Jun Lei⁴  Yu-Xia Qin²  Yi-Nan Zheng⁴  Xiang Li⁵ 

[1] School of Resources and Environment, Jilin Agricultural University, Changchun, 130118, China; E-mail:;Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University, Changchun, 130118, China; E-mail:;Institute of Agricultural Modernization, Jilin Agricultural University, Changchun, 130118, China; E-mails:;College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, 130118, China; E-mails:;Saskatoon Research Center, Agriculture and Agri-food Canada, Saskatoon, SK S7N 0X2, Canada

关键词: Aspergillus niger;    β-glucosidase;    ginsenoside Rf;    ginsenoside Rh₁;   

DOI  :  10.3390/molecules14062043

来源: mdpi

【 摘 要 】

An Aspergillus niger strain was isolated from the soil around ginseng fruit. In vitro enzyme assays showed that this strain had the ability to transform total ginsenosides (TGS) into several new products. In a further biochemical study, a β-glucosidase gene isolated from this strain, bgl1, was expressed in Saccharomyces cerevisiae. His-tagged BGL1 protein (~170 kD) showed the ability to transform ginsenoside Rf into Rh₁.

【 授权许可】

CC BY   
© 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.