Analysis of Intestinal Injuries Induced by Ricin in Vitro Using SPR Technology and MS Identification

International Journal of Molecular Sciences

Linna Liu¹  Hongwei Gao¹  Jiping Li¹  Ying Dong¹  Ning Liu¹  Jiayu Wan¹  Wensen Liu¹  Yucheng Sun¹ 

[1] Institute of Military Veterinary, Academy of Military Medical Science, Changchun 130062, China; E-Mails:

关键词: ricin;    BBM;    SPR;    HPCE-ESI-MS;   

DOI  :  10.3390/ijms10052431

来源: mdpi

【 摘 要 】

The present study found that ricin toxicity did not only manifest itself as inhibition of protein synthesis, but also induced apoptosis of immune cells and played an extremely significant role in intestinal injury. In this report, we describe a novel method to estimate binding events occurring on intestinal brush border membranes (BBM) based on SPR technology in an attempt to mimic the real intestinal surface capable of interacting physically and/or actively with certain biological molecules. Combined with HPCE-ESI-MS indentification, we obtained 28 kinds of proteins in BBM that interacted with ricin.

【 授权许可】

CC BY   
© 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.