Titration Calorimetry Standards and the Precision of Isothermal Titration Calorimetry Data

International Journal of Molecular Sciences

Lina Baranauskienė¹  Vilma Petrikaitė¹  Jurgita Matulienė¹ 

[1] Laboratory of Biothermodynamics and Drug Design / Institute of Biotechnology, Graičiūno 8, Vilnius, LT-02241, Lithuania

关键词: titration calorimetry;    enthalpy;    protein-ligand binding;    thermodynamics;    carbonic anhydrase;   

DOI  :  10.3390/ijms10062752

来源: mdpi

【 摘 要 】

Current Isothermal Titration Calorimetry (ITC) data in the literature have relatively high errors in the measured enthalpies of protein-ligand binding reactions. There is a need for universal validation standards for titration calorimeters. Several inorganic salt co-precipitation and buffer protonation reactions have been suggested as possible enthalpy standards. The performances of several commercial calorimeters, including the VP-ITC, ITC200, and Nano ITC-III, were validated using these suggested standard reactions.

【 授权许可】

CC BY   
© 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.