Sensors | |
Multivalent Anchoring and Oriented Display of Single-Domain Antibodies on Cellulose | |
Greg Hussack1  Yan Luo1  Linda Veldhuis2  J. Christopher Hall2  Jamshid Tanha1  | |
[1] Institute for Biological Sciences, National Research Council of Canada, 100 Sussex Drive, Ottawa, Ontario, Canada, K1A 0R6;Department of Environmental Biology, University of Guelph, Guelph, Ontario, Canada, N1G 2W1 | |
关键词:
single-domain antibody;
cellulose-binding module;
bispecific;
pentamer;
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DOI : 10.3390/s90705351 | |
来源: mdpi | |
【 摘 要 】
Antibody engineering has allowed for the rapid generation of binding agents against virtually any antigen of interest, predominantly for therapeutic applications. Considerably less attention has been given to the development of diagnostic reagents and biosensors using engineered antibodies. Recently, we produced a novel pentavalent bispecific antibody (i.e., decabody) by pentamerizing two single-domain antibodies (sdAbs) through the verotoxin B subunit (VTB) and found both fusion partners to be functional. Using a similar approach, we have engineered a bispecific pentameric fusion protein consisting of five sdAbs and five cellulose-binding modules (CBMs) linked via VTB. To find an optimal design format, we constructed six bispecific pentamers consisting of three different CBMs, fused to the
【 授权许可】
CC BY
© 2009 by the authors; licensee MDPI, Basel, Switzerland
【 预 览 】
Files | Size | Format | View |
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RO202003190056403ZK.pdf | 331KB | download |