From Lateral Flow Devices to a Novel Nano-Color Microfluidic Assay

Sensors

Saied Assadollahi¹  Christiane Reininger¹  Roland Palkovits¹  Peter Pointl¹ 

[1] Attophotonics Biosciences GmbH, Viktor Kaplan Strasse 2, A-2700 Wiener Neustadt, Austria; E-Mails:

关键词: lateral flow assays (LFAs);    resonance-enhanced absorption (REA);    AuNPs-bioconjugate;    polyethylene terephthalate (PET);    CO₂ laser ablation;   

DOI  :  10.3390/s90806084

来源: mdpi

【 摘 要 】

Improving the performance of traditional diagnostic lateral flow assays combined with new manufacturing technologies is a primary goal in the research and development plans of diagnostic companies. Taking into consideration the components of lateral flow diagnostic test kits; innovation can include modification of labels, materials and device design. In recent years, Resonance-Enhanced Absorption (REA) of metal nano-particles has shown excellent applicability in bio-sensing for the detection of a variety of bio-molecular binding interactions. In a novel approach, we have now integrated REA-assays in a diagnostic microfluidic setup thus resolving the bottleneck of long incubation times inherent in previously existing REA-assays and simultaneously integrated automated fabrication techniques for diagnostics manufacture. Due to the roller-coating based technology and chemical resistance, we used PET-co-polyester as a substrate and a CO₂ laser ablation system as a fast, highly precise and contactless alternative to classical micro-milling. It was possible to detect biological binding within three minutes – visible to the eye as colored text readout within the REA-fluidic device. A two-minute in-situ silver enhancement was able to enhance the resonant color additionally, if required.

【 授权许可】

CC BY   
© 2009 by the authors; licensee MDPI, Basel, Switzerland