Amperometric Hydrogen Peroxide Biosensor Based on Immobilization of Hemoglobin on a Glassy Carbon Electrode Modified with Fe3O4/Chitosan Core-Shell Microspheres

Sensors

Xue-Cai Tan¹  Jin-Lei Zhang²  Sheng-Wei Tan²  Dan-Dan Zhao²  Zen-Wei Huang²  Yan Mi² 

[1] College of Chemistry and Ecological Engineering, Guangxi University for Nationalities, Nanning 530006, China;

关键词: hemoglobin;    hydrogen peroxide;    magnetic microspheres;    biosensor;    hydroquinone;   

DOI  :  10.3390/s90806185

来源: mdpi

【 摘 要 】

Novel magnetic Fe₃O₄/chitosan (CS) microspheres were prepared using magnetic Fe₃O₄ nanoparticles and the natural macromolecule chitosan. Then, using an easy and effective hemoglobin (Hb) immobilization method, an innovative biosensor with a Fe₃O₄/CS-Hb-Fe₃O₄/CS “sandwich” configuration was constructed. This biosensor had a fast (less than 10 s) response to H₂O₂ and excellent linear relationships were obtained in the concentration range of 5.0 × 10⁻⁵ to 1.8 × 10⁻³ M and 1.8 × 10⁻³ to 6.8 × 10⁻³ M with a detection limit of 4.0 × 10⁻⁶ M (s/n = 3) under the optimum conditions. The apparent Michaelis-Menten constant K_m was 0.29 mM and it showed the excellent biological activity of the fixed Hb. Moreover, the biosensor had long-time stability and good reproducibility. The method was used to determine H₂O₂ concentration in real samples.

【 授权许可】

CC BY   
© 2009 by the authors; licensee MDPI, Basel, Switzerland