Variations in IC50 Values with Purity of Mushroom Tyrosinase

International Journal of Molecular Sciences

Elizabeth Neeley¹  George Fritch¹  Autumn Fuller¹  Jordan Wolfe¹  Jessica Wright¹ 

[1] id="af1-ijms-10-03811">Department of Chemistry and Physics, Indiana State University, Terre Haute, IN 47809, U

关键词: mushroom;    tyrosinase;    inhibitors;    IC₅₀ values;   

DOI  :  10.3390/ijms10093811

来源: mdpi

【 摘 要 】

The effects of various inhibitors on crude, commercial and partially purified commercial mushroom tyrosinase were examined by comparing IC₅₀ values. Kojic acid, salicylhydroxamic acid, tropolone, methimazole, and ammonium tetrathiomolybdate had relatively similar IC₅₀ values for the crude, commercial and partially purified enzyme. 4-Hexylresorcinol seemed to have a somewhat higher IC₅₀ value using crude extracts, compared to commercial or purified tyrosinase. Some inhibitors (NaCl, esculetin, biphenol, phloridzin) showed variations in IC₅₀ values between the enzyme samples. In contrast, hydroquinone, lysozyme, Zn²⁺, and anisaldehyde showed little or no inhibition in concentration ranges reported to be effective inhibitors. Organic solvents (DMSO and ethanol) had IC₅₀ values that were similar for some of the tyrosinase samples. Depending of the source of tyrosinase and choice of inhibitor, variations in IC₅₀ values were observed.

【 授权许可】

CC BY   
© 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.