A Novel Approach for Automated Analysis of Cell Attachment and Spreading Based on Backscattered Electron Imaging by Scanning Electron Microscopy

Materials

Alisa Katsen-Globa²  Leonora Peter²  Susan Zöllner²  Thomas Dörge²  Martin Daffertshofer¹  Hartwig Preckel¹  Daniel Schmitt² 

[1] Perkin Elmer Cellular Technologies GmbH, Schnackenburgallee 114, 22525 Hamburg, Germany; E-Mails:;Fraunhofer IBMT, Ensheimer Str.48, 66386 St.Ingbert, Germany; E-Mails:

关键词: cell adhesion;    cell spreading;    cytocompatibility of materials;    cell/material interactions;    scanning electron microscopy;    backscattered electron imaging;    image analysis;   

DOI  :  10.3390/ma2031402

来源: mdpi

【 摘 要 】

The development of new materials for biological application requires in vitro testing of cell/surface interactions. Cell adhesion and spreading are difficult to quantify as most materials are non-transparent and transmission microscopy cannot be used. Contrast in reflection microscopy is rather poor. We propose an alternative method for the automated screening of cell attachment and spreading using backscattered electron imaging of scanning electron microscopy. The enhanced cell contrast permits study of cell/material interactions by little differences between cells and material.

【 授权许可】

CC BY   
© 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.