Algorithm for the Analysis of Tryptophan Fluorescence Spectra and Their Correlation with Protein Structural Parameters

Algorithms

John Hixon¹ 

[1] Physics Department, University of Rhode Island, 2 Lippitt Rd, Kingston, RI 02881, USA; E-Mail

关键词: protein fluorescence;    protein structure;    decomposition;    structural analysis;    tryptophan;    statistical methods;   

DOI  :  10.3390/a2031155

来源: mdpi

【 摘 要 】

The fluorescence properties of tryptophan residues are sensitive to the microenvironment of fluorophores in proteins. Therefore, fluorescence characteristics are widely used to study structural transitions in proteins. However, the decoding of the structural information from spectroscopic data is challenging. Here we present a review of approaches developed for the decomposition of multi-component protein tryptophan fluorescence spectra and correlation of these spectral parameters with protein structural properties.

【 授权许可】

CC BY   
© 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.