Functional Analysis of a Putative Dothistromin Toxin MFS Transporter Gene

Toxins

Rosie E. Bradshaw¹  Zhilun Feng¹  Arne Schwelm¹  Yongzhi Yang² 

[1] Bio-Protection Research Centre, Institute of Molecular BioSciences, Massey University, Palmerston North, New Zealand;Biotechnology Research Institute, Qinghai Academy of Agriculture and Forestry, Xining, China;

关键词: aflatoxin biosynthesis;    Dothistroma septosporum;    major facilitator superfamily;    toxin transporter;    red-band needle blight;   

DOI  :  10.3390/toxins1020173

来源: mdpi

【 摘 要 】

Dothistromin is a non-host selective toxin produced by the pine needle pathogen Dothistroma septosporum. Dothistromin is not required for pathogenicity, but may have a role in competition and niche protection. To determine how D. septosporum tolerates its own toxin, a putative dothistromin transporter, DotC, was investigated. Studies with mutants lacking a functional dotC gene, overproducing DotC, or with a DotC-GFP fusion gene, did not provide conclusive evidence of a role in dothistromin efflux. The mutants revealed a major effect of DotC on dothistromin biosynthesis but were resistant to exogenous dothistromin. Intracellular localization studies suggest that compartmentalization may be important for dothistromin tolerance.

【 授权许可】

CC BY   
© 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland