On the Physical Meaning of the Isothermal Titration Calorimetry Measurements in Calorimeters with Full Cells

International Journal of Molecular Sciences

Jean-Pierre E. Grolier¹ 

[1] Laboratoire de Thermodynamique des Solutions et des Polymères Université Blaise Pascal, 24, Avenue des Landais, Clermont Ferrand, 63177 Aubière Cedex, France; E-Mail:

关键词: isothermal titration calorimetry;    ITC;    calorimetry;    thermodynamics;    infinite dilution;    binding;    partial properties;    enthalpy;    heat;   

DOI  :  10.3390/ijms10125296

来源: mdpi

【 摘 要 】

We have performed a detailed study of the thermodynamics of the titration process in an isothermal titration calorimeter with full cells. We show that the relationship between the enthalpy and the heat measured is better described in terms of the equation Δ H = W_inj + Q (where W_inj is the work necessary to carry out the titration) than in terms of ΔH = Q. Moreover, we show that the heat of interaction between two components is related to the partial enthalpy of interaction at infinite dilution of the titrant component, as well as to its partial volume of interaction at infinite dilution.

【 授权许可】

CC BY   
© 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.