On-Chip Immunoassay for Determination of Urinary Albumin

Sensors

Wanida Laiwattanapaisal¹  Temsiri Songjaroen²  Thitima Maturos³  Tanom Lomas³  Assawapong Sappat³ 

[1] Centre for Excellence in Omics-Nano Medical Technology Project Development, Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, 10330, Thailand;Graduate Programme in Clinical Biochemistry and Molecular Medicine, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, 10330, Thailand; E-Mail:;Nanoelectronics and MEMS Laboratory, National Electronics and Computer Technology Centre, Pathum Thani, 12120, Thailand; E-Mails:

关键词: microalbuminuria;    microfluidic system;    on-chip immunoassay;    poly(dimethylsiloxane);   

DOI  :  10.3390/s91210066

来源: mdpi

【 摘 要 】

An immunoassay performed on a portable microfluidic device was evaluated for the determination of urinary albumin. An increase in absorbance at 500 nm resulting from immunoagglutination was monitored directly on the poly(dimethylsiloxane) (PDMS) microchip using a portable miniature fibre-optic spectrometer. A calibration curve was linear up to 10 mg L^–1 (r² = 0.993), with a detection limit of 0.81 mg L^–1 (S/N = 3). The proposed system showed good precision, with relative standard deviations (RSDs) of 5.1%, when evaluated with 10 mg L^–1 albumin (n = 10). Determination of urinary albumin with the proposed system gave results highly similar to those determined by the conventional spectrophotometric method using immunoturbidimetric detection (r² = 0.995; n = 15).

【 授权许可】

CC BY   
© 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.