Viral Hybrid Vectors for Somatic Integration - Are They the Better Solution?

Viruses

Nadine Müther¹  Nadja Noske¹ 

[1] >Max von Pettenkofer-Institut, Department of Virology, Ludwig-Maximilians-Universität Munich, Pettenkoferstr. 9A, 80336 Munich, Germa

关键词: hybrid vector;    somatic integration;    adenovirus;    retrovirus;    adeno-associated virus;    herpes simplex virus;    targeted integration;    Sleeping Beauty transposase;    PhiC31 integrase;    zinc-finger nuclease;   

DOI  :  10.3390/v1031295

来源: mdpi

【 摘 要 】

The turbulent history of clinical trials in viral gene therapy has taught us important lessons about vector design and safety issues. Much effort was spent on analyzing genotoxicity after somatic integration of therapeutic DNA into the host genome. Based on these findings major improvements in vector design including the development of viral hybrid vectors for somatic integration have been achieved. This review provides a state-of-the-art overview of available hybrid vectors utilizing viruses for high transduction efficiencies in concert with various integration machineries for random and targeted integration patterns. It discusses advantages but also limitations of each vector system.

【 授权许可】

CC BY   
© 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland