HIV-1 Integrase-DNA Recognition Mechanisms

Viruses

Jacques J. Kessl¹  Christopher J. McKee¹  Jocelyn O. Eidahl¹  Nikolozi Shkriabai¹  AriKatz¹ 

[1] >Center for Retrovirus Research and Comprehensive Cancer Center, College of Pharmacy, The Ohio State University, Columbus, OH 43210, USA; E-Mails: [e-mail] (J.J.K.); [e-mail] (C.J.M.); [e-mail] (J.O.E.), [e-mail] (N.S.); [e-mail] (A.K

关键词: HIV;    retroviruses;    DNA;    integrase;    crosslinking;    footprinting;   

DOI  :  10.3390/v1030713

来源: mdpi

【 摘 要 】

Integration of a reverse transcribed DNA copy of the HIV viral genome into the host chromosome is essential for virus replication. This process is catalyzed by the virally encoded protein integrase. The catalytic activities, which involve DNA cutting and joining steps, have been recapitulated in vitro using recombinant integrase and synthetic DNA substrates. Biochemical and biophysical studies of these model reactions have been pivotal in advancing our understanding of mechanistic details for how IN interacts with viral and target DNAs, and are the focus of the present review.

【 授权许可】

CC BY   
© 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland