Violacein-Producing Collimonas sp. from the Sea Surface Microlayer of Costal Waters in Trøndelag, Norway

Marine Drugs

Sigrid Hakvåg²  Espen Fjærvik²  Geir Klinkenberg¹  Sven Even F. Borgos²  Kjell D. Josefsen¹  Trond E. Ellingsen¹ 

[1] SINTEF Industrial Biotechnology, SINTEF, N-7034 Trondheim, Norway; E-Mails:;Department of Biotechnology, Norwegian University of Science and Technology, N-7491 Trondheim, Norway; E-Mails:

关键词: surface microlayer;    Collimonas;    violacein;    biosynthetic genes;   

DOI  :  10.3390/md7040576

来源: mdpi

【 摘 要 】

A new strain belonging to the genus Collimonas was isolated from the sea surface microlayer off the coast of Trøndelag, Norway. The bacterium, designated Collimonas CT, produced an antibacterial compound active against Micrococcus luteus. Subsequent studies using LC-MS identified this antibacterial compound as violacein, known to be produced by several marine-derived bacteria. Fragments of the violacein biosynthesis genes vioA and vioB were amplified by PCR from the Collimonas CT genome and sequenced. Phylogenetic analysis of these sequences demonstrated close relatedness of the Collimonas CT violacein biosynthetic gene cluster to those in Janthinobacterium lividum and Duganella sp., suggesting relatively recent horizontal gene transfer. Considering diverse biological activities of violacein, Collimonas CT shall be further studied as a potential producer of this compound.

【 授权许可】

CC BY   
© 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland