High-Performance Liquid Chromatographic Quantification of Flavonoids in Eriocaulaceae Species and Their Antimicrobial Activity

Molecules

Marcelo Aparecido Da Silva¹  Claudia Andréa Lima Cardoso¹  Wagner Vilegas¹ 

[1] ¹UNESP-Univ Estadual Paulista, Instituto de Química, Departamento de Química Orgânica, CP 355, CEP 14800-900, Araraquara, São Paulo, Brazil ²UEMS, Course of Chemistry, Mato Grosso do Sul State University, CP 351, CEP 79804-970, Dourados, Mato Grosso do Sul, Brazil

关键词: eriocaulaceae;    liquid chromatography;    quantification;    flavonoids;   

DOI  :  10.3390/molecules14114644

来源: mdpi

【 摘 要 】

Quantification of prepared samples by analysis using high performance liquid chromatography with DAD detection was developed to analyze rutin, 6-methoxyapigenin, and 6-methoxyapigenin-7-O-β-D-glucopyranoside isolated from methanolic extracts of different genus: Syngonanthus, Leiothix and Eriocaulon (Eriocaulaceae). The linearity, accuracy, and the inter-day precision of the procedure were evaluated. The calibration curves were linear. The recoveries of the flavonoids in the samples analyzed were 96.3% to 98.5%. The percentage coefficient of variation for the quantitative analysis of the flavonoids in the analyses of the samples was under 5%. The antimicrobial activity of the five methanol extracts of these Eriocaulaceae species was assayed against the microorganisms Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Salmonella setubal, Saccharomyces cerevisiae and Candida albicans. Measured MIC values ranged from 1.25 to 10.00 mg/mL. The flavonoid contents suggest that Eriocaulaceae species may be a promising source of compounds to produce natural phytomedicines.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.