A Novel Biosensor for Evaluation of Apoptotic or Necrotic Effects of Nitrogen Dioxide during Acute Pancreatitis in Rat

Sensors

Dagmara Jacewicz³  Aleksandra Dabrowska³  Dariusz Wyrzykowski³  Joanna Pranczk³  Michal Wozniak²  Jolanta Kubasik-Juraniec¹  Narcyz Knap²  Kamila Siedlecka⁴  Alexander J. Neuwelt⁵ 

[1] Department of Electron Microscopy, Medical University of Gdańsk, Dębinki 1, 80-211, Gdańsk, Poland; E-Mail:;Department of Medical Chemistry, Medical University of Gdańsk, Dębinki 1, 80-211, Gdańsk, Poland; E-Mails:;Department of General and Inorganic Chemistry, University of Gdańsk, Sobieskiego 18/19, 80-952 Gdańsk, Poland; E-Mails:;Department of Histology & Immunology, Medical University of Gdańsk, Dębinki 1, 80-211, Gdańsk, Poland; E-Mail:;Department of Neurology, Oregon Health & Sciences University, Portland OR 97239, USA; E-Mail:

关键词: acute pancreatitis;    nitrogen dioxide;    l-arginine;    Cr(III);    rats;    levels of inflammatory mediators;    evaluation of apoptotic or necrotic effects;   

DOI  :  10.3390/s100100280

来源: mdpi

【 摘 要 】

The direct and accurate estimation of nitric dioxide levels is an extremely laborious and technically demanding procedure in the molecular diagnostics of inflammatory processes. The aim of this work is to demonstrate that a stop-flow technique utilizing a specific spectroscopic biosensor can be used for detection of nanomolar quantities of NO₂ in biological milieu. The use of novel compound cis-[Cr(C₂O₄)(AaraNH₂)(OH₂)₂]⁺ increases NO₂ estimation accuracy by slowing down the rate of NO₂ uptake. In this study, an animal model of pancreatitis, where nitrosative stress is induced by either 3g/kg bw or 1.5 g/kg bw dose of l-arginine, was used. Biochemical parameters and morphological characteristics of acute pancreatitis were monitored, specifically assessing pancreatic acinar cell death mode, NO₂ generation and cellular glutathione level. The severity of the process correlated positively with NO₂ levels in pancreatic acinar cell cytosol samples, and negatively with cellular glutathione levels.

【 授权许可】

CC BY   
©2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.