Molecular Polymorphisms in Tunisian Pomegranate (Punica granatum L.) as Revealed by RAPD Fingerprints

Diversity

Néjib Hasnaoui¹  Messaoud Mars¹  Jemni Chibani² 

[1] U.R. Agro-biodiversity, Higher Agronomic Institute, 4042 Chott Mariem, Sousse, Tunisia; E-Mail:;Laboratory of Biochemistry & Molecular Biology, Faculty of Pharmacy, 5019 Monastir, Tunisia; E-Mail:

关键词: pomegranate;    Punica granatum L.;    diversity;    RAPD;   

DOI  :  10.3390/d2010107

来源: mdpi

【 摘 要 】

The genetic diversity among Tunisian pomegranate cultivars has been investigated. Using universal primers, the random amplified polymorphic DNA (RAPD) method was used to generate banding profiles from a set of twelve cultivars. Data was then computed with appropriate programs to construct a dendrogram illustrating the relationships between the studied cultivars. Our data proved the efficiency of the designed method to examine the DNA polymorphism in this crop since the tested primers are characterized by a collective resolving power of 12.83. In addition, the cluster analysis has exhibited a parsimonious tree branching independent from the geographic origin of the cultivars. In spite of the relatively low number of primers and cultivars, RAPD constitutes an appropriate procedure to assess the genetic diversity and to survey the phylogenetic relationships in this crop.

【 授权许可】

CC BY   
© 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.