Melatonin and Structurally-Related Compounds Protect Synaptosomal Membranes from Free Radical Damage

International Journal of Molecular Sciences

Sergio Millán-Plano¹  Eduardo Piedrafita¹  Francisco J. Miana-Mena¹  Lorena Fuentes-Broto¹  Enrique Martínez-Ballarín¹  Laura López-Pingarrón²  Mar໚ A. Sพnz¹ 

[1]Department of Pharmacology and Physiology, Faculty of Medicine, University of Zaragoza, C/Domingo Miral s/n, 50009, Zaragoza, Spain

[2] E-Mails:

[3]Department of Human Anatomy and Histology, Faculty of Medicine, University of Zaragoza, C/Domingo Miral s/n, 50009, Zaragoza, Spain

[4] E-Mail:

关键词: melatonin;    pinoline;    indoleamine;    free radical;    lipid peroxidation;    protein oxidation;    synaptosome;   

DOI  :  10.3390/ijms11010312

来源: mdpi

【 摘 要 】

Since biological membranes are composed of lipids and proteins we tested the in vitro antioxidant properties of several indoleamines from the tryptophan metabolic pathway in the pineal gland against oxidative damage to lipids and proteins of synaptosomes isolated from the rat brain. Free radicals were generated by incubation with 0.1 mM FeCl₃, and 0.1 mM ascorbic acid. Levels of malondialdehyde (MDA) plus 4-hydroxyalkenal (4-HDA), and carbonyl content in the proteins were measured as indices of oxidative damage to lipids and proteins, respectively. Pinoline was the most powerful antioxidant evaluated, with melatonin, N-acetylserotonin, 5-hydroxytryptophan, 5-methoxytryptamine, 5-methoxytryptophol, and tryptoline also acting as antioxidants.

【 授权许可】

CC BY   
© 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.