Toxins | |
PP2A Inhibition Assay Using Recombinant Enzyme for Rapid Detection of Okadaic Acid and Its Analogs in Shellfish | |
Tsuyoshi Ikehara1  Shihoko Imamura1  Atsushi Yoshino1  | |
[1] Tropical Technology Center Ltd., 5-1 Suzaki, Uruma, Okinawa 904-2234, Japan; | |
关键词: diarrhetic shellfish poisoning (DSP); okadaic acid (OA); protein phosphatase 2A (PP2A) inhibition assay; digestive gland (DG); | |
DOI : 10.3390/toxins2010195 | |
来源: mdpi | |
【 摘 要 】
Okadaic acid and its analogs (OAs) responsible for diarrhetic shellfish poisoning (DSP) strongly inhibit protein phosphatase 2A (PP2A) and thus are quantifiable by measuring the extent of the enzyme inhibition. In this study, we evaluated the suitability of the catalytic subunit of recombinant human PP2A (rhPP2Ac) for use in a microplate OA assay. OA, dinophysistoxin-1(DTX1), and hydrolyzate of 7-
【 授权许可】
CC BY
© 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland
【 预 览 】
Files | Size | Format | View |
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RO202003190054919ZK.pdf | 324KB | download |