The Solubility of Hen Lysozyme in Ethylammonium Nitrate/H2O Mixtures and a Novel Approach to Protein Crystallization

Molecules

Nolene Byrne¹ 

[1] Department of Chemistry and Biochemistry, Arizona State University, Tempe 85287, AZ, USA ^†Current address: Centre for Materials and Fibre Innovation, Institute for Technology Research & Innovation, Deakin University, Geelong, Victoria 3217, Australia

关键词: protic ionic liquids;    protein crystallization;    solubility and protein stability;   

DOI  :  10.3390/molecules15020793

来源: mdpi

【 摘 要 】

We report on the solubility of hen lysozyme (HEWL) in aqueous ethylammonium nitrate (EAN) as a function of water content. We find the solubility behavior to be complex, exhibiting both a maximum (400 mg/mL) at very high EAN content) and a minimum at intermediate EAN content. We exploit this solubility profile in a novel approach to generating crystals of hydrophilic proteins, based on rehydration of a high concentration protein solution. We describe the production of crystals of X-ray diffraction quality. Two related ionic liquid solvent systems, with the same solubility profiles but different effective pH characteristics, are identified for future evaluation.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.