A Novel System for Identification of Inhibitors of Rift Valley Fever Virus Replication

Viruses

Mary E. Piper¹ 

[1] Cellular and Molecular Biology Program, University of Michigan, Ann Arbor, MI, USA;E-Mail: [e-mail] (M.E.P.)

关键词: Rift Valley fever;    virus-like particle;    RNA virus;    Bunyaviridae;    Phlebovirus;    minigenome;    antivirals;   

DOI  :  10.3390/v2030731

来源: mdpi

【 摘 要 】

Rift Valley fever virus (RVFV) is a human and livestock pathogen endemic to sub-Saharan Africa. We have developed a T7-dependent system for the efficient production of RVFV-like particles (RVF-VLPs) based on the virulent ZH-501 strain of RVFV. The RVF-VLPs are capable of performing a single round of infection, allowing for the study of viral replication, assembly, and infectivity. We demonstrate that these RVF-VLPs are antigenically indistinguishable from authentic RVFV and respond similarly to a wide array of known and previously unknown chemical inhibitors. This system should be useful for screening for small molecule inhibitors of RVFV replication.

【 授权许可】

CC BY   
© 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland