Hitherto Unrecognized Fluorescence Properties of Coniferyl Alcohol

Molecules

Komandoor Elayavalli Achyuthan¹  Paul David Adams¹  Supratim Datta¹  Blake Alexander Simmons¹ 

[1] ¹Joint BioEnergy Institute (JBEI), Emeryville, CA 94550, USA ²Biosensors and Nanomaterials Department, Sandia National Laboratories, Albuquerque, NM 87185, USA ³Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA ⁴Sandia National Laboratories, Livermore, CA 94550, USA

关键词: coniferyl alcohol;    fluorescence;    laccase;    peroxidase;    high throughput screening;    lignin;    biofuels;   

DOI  :  10.3390/molecules15031645

来源: mdpi

【 摘 要 】

We instituted a quasi-quality assurance program for demonstrating coniferyl alcohol’s fluorescence and fluorescence diminishment following enzymatic oxidation. The magnitude of diminishment was a measure of catalysis. High throughput screening was performed in pseudo-kinetic and endpoint modes by measuring the fluorescence at 416 nm following excitation at 290, 310 or 340 nm. Dose-response tracings were linear between two and three orders of magnitude with average limits of detection and quantitation of 1.8 and 6.9 mM coniferyl alcohol, respectively. Oxidation was evident with 0.025 mg/mL laccase or 0.003 mg/mL peroxidase or inside 5 min using 0.5 mg/mL laccase or 5 mM substrate. Sodium chloride inhibited (IC₅₀, 25 mM) laccase oxidation of coniferyl alcohol. Fluorescence from 10 concentrations (1 to 1000 mM) of coniferyl alcohol was stable for 24 hours over 14 excitation/emission cycles at 3 different combinations of excitation and emission wavelengths. In conclusion, coniferyl alcohol absorption and fluorescence assays should facilitate biomass lignin analyses and improve delignification.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.