Chromatin Fiber Dynamics under Tension and Torsion

International Journal of Molecular Sciences

Christophe Lavelle²  Jean-Marc Victor¹ 

[1] Laboratoire de Physique Théorique de la Matière Condensée, CNRS UMR 7600, Paris F-75005, France;Interdisciplinary Research Institute, CNRS USR 3078, Villeneuve d'Ascq F-59655, France

关键词: DNA;    nucleosome;    chromatin;    single molecule;    magnetic tweezers;    optical tweezers;   

DOI  :  10.3390/ijms11041557

来源: mdpi

【 摘 要 】

Genetic and epigenetic information in eukaryotic cells is carried on chromosomes, basically consisting of large compact supercoiled chromatin fibers. Micromanipulations have recently led to great advances in the knowledge of the complex mechanisms underlying the regulation of DNA transaction events by nucleosome and chromatin structural changes. Indeed, magnetic and optical tweezers have allowed opportunities to handle single nucleosomal particles or nucleosomal arrays and measure their response to forces and torques, mimicking the molecular constraints imposed in vivo by various molecular motors acting on the DNA. These challenging technical approaches provide us with deeper understanding of the way chromatin dynamically packages our genome and participates in the regulation of cellular metabolism.

【 授权许可】

CC BY   
© 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.