Transcellular Transport of Heparin-coated Magnetic Iron Oxide Nanoparticles (Hep-MION) Under the Influence of an Applied Magnetic Field

Pharmaceutics

Kyoung Ah Min¹  Faquan Yu¹  Victor C. Yang¹  Xinyuan Zhang¹ 

[1] Department of Pharmaceutical Sciences, College of Pharmacy, University of Michigan, 428 Church St., Ann Arbor, MI 48109, USA; E-Mails:

关键词: magnetic iron oxide nanoparticles (MION);    magnetic field;    transcellular transport;    MDCK cell monolayer;    drug targeting;   

DOI  :  10.3390/pharmaceutics2020119

来源: mdpi

【 摘 要 】

In this study, magnetic iron oxide nanoparticles coated with heparin (Hep-MION) were synthesized and the transcellular transport of the nanoparticles across epithelial cell monolayers on porous polyester membranes was investigated. An externally applied magnetic field facilitated the transport of the Hep-MION across cell monolayers. However, high Hep-MION concentrations led to an increased aggregation of nanoparticles on the cell monolayer after application of the magnetic field. Our results indicate that magnetic guidance of Hep-MION most effectively promotes transcellular transport under conditions that minimize formation of magnetically-induced nanoparticle aggregates. Across cell monolayers, the magnet’s attraction led to the greatest increase in mass transport rate in dilute dispersions and in high serum concentrations, suggesting that magnetic guidance may be useful for in vivo targeting of Hep-MION.

【 授权许可】

CC BY   
© 2010 by the authors; licensee MDPI, Basel, Switzerland.