Oak (Quercus frainetto Ten.) Honeydew Honey—Approach to Screening of Volatile Organic Composition and Antioxidant Capacity (DPPH and FRAP Assay)

Molecules

Igor Jerković¹ 

[1] ¹Faculty of Chemistry and Technology, University of Split, N. Tesle 10/V, 21000 Split, Croatia ²Marko Marulić Polytechnic in Knin, P. Krešimira IV 30, 22300 Knin, Croatia

关键词: Quercus frainetto Ten. honeydew honey;    headspace solid-phase microextraction (HS-SPME);    ultrasonic solvent extraction (USE);    gas chromatography and mass spectrometry (GC and GC/MS);    DPPH and FRAP assay;   

DOI  :  10.3390/molecules15053744

来源: mdpi

【 摘 要 】

Two samples of oak honeydew honey were investigated. Headspace solid-phase microextraction (HS-SPME) combined with GC and GC/MS enabled identification of the most volatile organic headspace compounds being dominated by terpenes(mainly cis- and trans-linalool oxides). The volatile and less-volatile organic composition of the samples was obtained by ultrasonic assisted extraction (USE) with two solvents (1:2 (v/v) pentane -diethyl ether mixture and dichloromethane) followed by GC and GC/MS analysis. Shikimic pathway derivatives are of particular interest with respect to the botanical origin of honey and the most abundant was phenylacetic acid (up to 16.4%). Antiradical activity (DPPH assay) of the honeydew samples was 4.5 and 5.1 mmol TEAC/kg. Ultrasonic solvent extracts showed several dozen times higher antiradical capacity in comparison to the honeydew. Antioxidant capacity (FRAP assay) of honeydew samples was 4.8 and 16.1 mmol Fe²⁺/kg, while the solvent mixture extracts showed antioxidant activity of 374.5 and 955.9 Fe²⁺/kg, respectively, and the dichloromethane extracts 127.3 and 101.5 mmol Fe²⁺/kg.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.