Next Generation DNA Sequencing and the Future of Genomic Medicine

Genes

Matthew W. Anderson¹ 

[1] Department of Pathology, Stanford University Medical Center, 300 Pasteur Drive, Room L235, Stanford, CA 94305-5627, USA; E-Mail:

关键词: DNA;    sequencing;    next generation sequencing;    bioinformatics;    molecular diagnostics;   

DOI  :  10.3390/genes1010038

来源: mdpi

【 摘 要 】

In the years since the first complete human genome sequence was reported, there has been a rapid development of technologies to facilitate high-throughput sequence analysis of DNA (termed “next-generation” sequencing). These novel approaches to DNA sequencing offer the promise of complete genomic analysis at a cost feasible for routine clinical diagnostics. However, the ability to more thoroughly interrogate genomic sequence raises a number of important issues with regard to result interpretation, laboratory workflow, data storage, and ethical considerations. This review describes the current high-throughput sequencing platforms commercially available, and compares the inherent advantages and disadvantages of each. The potential applications for clinical diagnostics are considered, as well as the need for software and analysis tools to interpret the vast amount of data generated. Finally, we discuss the clinical and ethical implications of the wealth of genetic information generated by these methods. Despite the challenges, we anticipate that the evolution and refinement of high-throughput DNA sequencing technologies will catalyze a new era of personalized medicine based on individualized genomic analysis.

【 授权许可】

CC BY   
© 2010 by the authors; licensee MDPI, Basel, Switzerland