Isolation and Characterization of Novel Microsatellite Markers in Pomegranate (Punica granatum L.)

International Journal of Molecular Sciences

Seyed Mostafa Pirseyedi²  Sahar Valizadehghan²  Mohsen Mardi²  Mohammad Reza Ghaffari²  Parvaneh Mahmoodi²  Mehdi Zahravi¹  Mehrshad Zeinalabedini² 

[1] Iranain National Gene Bank, Mahdasht Road, Karaj, Iran; E-Mail:;Department of Genomics, Agricultural Biotechnology Research Institute of Iran, Mahdasht Road, Karaj, Iran; E-Mails:

关键词: Punica granatum;    microsatellite;    pomegranate;    SSR;   

DOI  :  10.3390/ijms11052010

来源: mdpi

【 摘 要 】

Pomegranate (Punica granatum L.) has been cultivated from ancient times for its economic, ornamental and medicinal properties globally. Here, we report the isolation and characterization of 12 polymorphic microsatellite markers from a repeat-enriched genomic library of Punica granatum L. The genetic diversity of these loci was assessed in 60 genotypes of Punica granatum L. All loci were variable: the number of polymorphic alleles per locus ranged from two to five (average 2.9). The observed and expected heterozygosities ranged from 0.15 to 0.87 and 0.29 to 0.65, respectively. The polymorphic information content ranged from 0.26 to 0.61 (average: 0.43). To the best of our knowledge, this is the first time that polymorphic microsatellite markers have been reported for P. granatum L. These new markers should allow studies of the population structure and genetic diversity of pomegranate to be performed in the future.

【 授权许可】

CC BY   
© 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.