Biosensing for the Environment and Defence: Aqueous Uranyl Detection Using Bacterial Surface Layer Proteins

Sensors

David J.R. Conroy¹  Paul A. Millner¹  Douglas I. Stewart² 

[1] Biosensors and Biocatalysis Group, Institute of Membranes and Systems Biology, University of Leeds, Leeds, LS2 9JT, UK; E-Mail:;School of Civil Engineering, University of Leeds, Leeds LS2 9JT, UK; E-Mail:

关键词: S-layer;    surface layer;    protein biosensor;    uranium;    uranyl;    metal ion;    sequestering;    impedance spectroscopy;   

DOI  :  10.3390/s100504739

来源: mdpi

【 摘 要 】

The fabrication of novel uranyl (UO₂²⁺) binding protein based sensors is reported. The new biosensor responds to picomolar levels of aqueous uranyl ions within minutes using Lysinibacillus sphaericus JG-A12 S-layer protein tethered to gold electrodes. In comparison to traditional self assembled monolayer based biosensors the porous bioconjugated layer gave greater stability, longer electrode life span and a denser protein layer. Biosensors responded specifically to UO₂²⁺ ions and showed minor interference from Ni²⁺, Cs⁺, Cd²⁺ and Co²⁺. Chemical modification of JG-A12 protein phosphate and carboxyl groups prevented UO₂²⁺ binding, showing that both moieties are involved in the recognition to UO₂²⁺.

【 授权许可】

CC BY   
© 2010 by the authors; licensee MDPI, Basel, Switzerland.