Characterization of the Active Constituents in Shixiao San Using Bioactivity Evaluation Followed by UPLC-QTOF and Markerlynx Analysis

Molecules

Wei Zhou¹  Shu-Lan Su¹  Jin-Ao Duan¹  Jian-Ming Guo¹  Da-Wei Qian¹  Er-Xin Shang¹ 

[1] Jiangsu Key laboratory for TCM Formulae Research (Supported by the Cyrus Tang Foundation of USA); Nanjing University of Chinese Medicine, Nanjing 210046, China

关键词: Shixiao San;    analgesic activity;    active components;    UPLC-QTOF-MS;    Markerlynx^TM;   

DOI  :  10.3390/molecules15096217

来源: mdpi

【 摘 要 】

Shixiao San is a famous Traditional Chinese Medicine (TCM) formula that has been used for a long time for the treatment of gynecological diseases. In this paper, the active constituents in Shixiao San were characterized by using bioactivity evaluation followed by UPLC-QTOF and Markerlynx^TM analysis. The analgesic activities of two extracts of Shixiao San were evaluated using the hot-plate test, acetic acid-induced writhing and dysmenorrhea mice model. The results showed that the analgesic activity of Shixiao San vinegary extract (boiling vinegar) was superior to the aqueous extract. UPLC-QTOF and Markerlynx^TM analytic results showed that the process of boiling in vinegar may improve the dissolution of Shixiao San flavanoids, and these flavanoids may contribute to the observed analgesic activity. This work demonstrated that UPLC/QTOFMS and Markerlynx^TM could serve as new methods for fast generation and automated analysis of information-rich data from Chinese herbal medicines.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.